evaluation of mir-141, mir-200c, mir-30b expression and clinicopathological features of bladder cancer

نویسندگان

ali mahdavinezhad department of genetics and molecular medicine ,hamedan university of medical sciences, hamedan, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (hamadan university of medical sciences)

seyed habibollah mousavi-bahar shaheed beheshti hospital, hamedan university of medical sciences, hamadan, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (hamadan university of medical sciences)

jalal poorolajal department of epidemiology & biostatistics, school of public health, hamedan university of medical sciences, hamedan, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (hamadan university of medical sciences)

reza yadegarazar department of genetics and molecular medicine ,hamedan university of medical sciences, hamedan, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی همدان (hamadan university of medical sciences)

چکیده

bladder cancer (bc) ranks the second most common genitourinary tract malignant tumor with high mortality and 70% recurrence rate worldwide. mirnas expression has noticeable role in bladder tumorigenesis. the purpose of this study was to assess mir-200c, mir-30b and mir-141 in tissue samples of patients with bc and healthy adjacent tissue samples and their association with muscle invasion, grade and the size of the tumor. transurethral resection tissue samples were collected from thirty- five newly diagnosed untreated patients with bc from 2013 to 2014. the control group consisted of adjacent normal urothelium. all samples, observed by two pathologists, were diagnosed transitional cell carcinomas (tcc) with the proportion of tumor cells greater than 80%. total rna including mirnas was extracted from about 50 mg tissue samples by applying trizol reagent. 2(-δδ ct) method was used to calculate relative quantification of mirna expression. two of 35 patients were females and the other 33 were males. invasion to bladder muscle was observed in 23 (67%) cases. mir-141, mir-200-c and mir30-b were up-regulated in 91%, 79% and 64% of malignant tissues, respectively. down-regulation of mir-141 had a strong association with muscle invasion (p= 0.017). significant inverse correlation between grading and mirna-141 level was observed (p= 0.043).

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of miR-141, miR-200c, miR-30b Expression and Clinicopathological Features of Bladder Cancer

Bladder cancer (BC) ranks the second most common genitourinary tract malignant tumor with high mortality and 70% recurrence rate worldwide. MiRNAs expression has noticeable role in bladder tumorigenesis. The purpose of this study was to assess miR-200c, miR-30b and miR-141 in tissue samples of patients with BC and healthy adjacent tissue samples and their association with muscle invasion, grade...

متن کامل

Evaluation of miR-141, miR-200c, miR-30b Expression and Clinicopathological Features of Bladder Cancer

Bladder cancer (BC) ranks the second most common genitourinary tract malignant tumor with high mortality and 70% recurrence rate worldwide. MiRNAs expression has noticeable role in bladder tumorigenesis. The purpose of this study was to assess miR-200c, miR-30b and miR-141 in tissue samples of patients with BC and healthy adjacent tissue samples and their association with muscle invasion, grade...

متن کامل

Association between tissue miR-141, miR-200c and miR-30b and bladder cancer: a matched case-control study.

PURPOSE To evaluate the expression of microRNAs in tissue samples from patients with bladder cancer and to compare it with healthy adjacent tissue samples as controls. MATERIALS AND METHODS Thirty five tissue samples from patients with newly diagnosed untreated bladder transitional cell carcinoma and 35 adjacent normal urothelium were collected during 2013 to 2014. TRIzol reagent was used to ...

متن کامل

Regulation of miR‐200c and miR‐141 by Methylation in Prostate Cancer

BACKGROUND In prostate cancer (PCa), abnormal expression of several microRNAs (miRNAs) has been previously reported. Increasing evidence shows that aberrant epigenetic regulation of miRNAs is a contributing factor to their altered expression in cancer. In this study, we investigate whether expression of miR-200c and miR-141 in PCa is related to the DNA methylation status of their promoter. ME...

متن کامل

Correlation analysis of miR-200b, miR-200c, and miR-141 with liver metastases in colorectal cancer patients.

OBJECTIVE To investigate correlation relationship between serum miR-200b, miR-200c, miR-141 levels with liver metastases in colorectal cancer patients. PATIENTS AND METHODS A total of 85 colorectal cancer patients with liver metastases and 54 colorectal cancer patients without liver metastases were divided into experimental group and control. Serum sample was collected before surgery and test...

متن کامل

Role for DNA Methylation in the Regulation of miR-200c and miR-141 Expression in Normal and Cancer Cells

BACKGROUND The microRNA-200 family participates in the maintenance of an epithelial phenotype and loss of its expression can result in epithelial to mesenchymal transition (EMT). Furthermore, the loss of expression of miR-200 family members is linked to an aggressive cancer phenotype. Regulation of the miR-200 family expression in normal and cancer cells is not fully understood. METHODOLOGY/P...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of molecular and cellular medicine

جلد ۴، شماره ۱، صفحات ۳۲-۳۹

کلمات کلیدی
[ ' u r i n a r y b l a d d e r n e o p l a s m ' , ' m i c r o r n a ' , ' 2 0 0 c ' , ' m i c r o r n a ' , 1 4 1 , ' m i c r o r n a ' , ' 3 0 b ' , ' n e o p l a s m g r a d i n g ' , ' n e o p l a s m i n v a s i v e n e s s ' ]

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023